Pre-basic seed potato (Solanum tuberosum L.) production using temporary immersion bioreactors
DOI:
https://doi.org/10.21704/pja.v2i1.1127Keywords:
Microtubers, temporary immersion bioreactor, pre-basic seed, microtuberization.Abstract
Potato is an important food and cash crop in Perú. Its production is limited due to seed quality, and other biotic and abiotic factors. Here we explore a new alternative for potato pre-basic seed production named temporary immersion bioreactor (TIB). Potato microtubers production using TIB comprises two phases: multiplication and microtuberization. During the multiplication phase, we used 50 segments of four nodes from a semi-solid medium with an immersion and frequency time of five minutes and three hours, respectively. In addition, a photoperiod of 16 hours of light and eight hours of darkness during 28 days were employed. For the microtuberization phase and under darkness conditions during 60 days, we used 80 g/l of sucrose and 30 ml/explant as a volume for the medium. Then, it was possible to obtain five, six, and 2.5 microtubers per explant of potato varieties “Canchan INIA”, “Capiro”, and “Papa 3”, respectively.Downloads
References
Coleman W.K., Donnelly, D.J. and Coleman S.E. (2001). Potato microtubers as research tools. A Review. American Journal of Potato Research 78(1): 47-55. DOI: 10.1007/BF02874824
Désiré, S., Couillerot, J.P. and Vasseur. J. (1995). Dormancy and sprouting of in vitro potato (Solanum tuberosum L.) microtubers: Effects of sucrose concentration from tuberization medium, storage period at 4◦C and gibberellic acid treatment. Acta Botanica Gallica 142: 371-378.
Dobranski, J., Magyar-Tabori, K. and Hudak. H. (2008). In vitro tuberization in hormone-free systems on solidified medium and dormancy of potato microtubers. London: Global Science Books.
Donnelly, D.J., Coleman, W.K. and Coleman. S.E. (2003). Potato microtuber production and performance. A review. American Journal Potato Research 80(2): 103-115. https://doi.org/10.1007/BF02870209
Espinoza, N.O., Estrada, R., Silva-Rodríguez, D., Tovar, P., Lizzaraga, R., and Dodds. J.H. (1986). The potato: A model crop plant for tissue culture. Outlook on Agriculture 15(1): 21-26. https://doi.org/10.1177/003072708601500104
Gopal, J., Minocha, J.L. and Dhaliwal H.S. (1998). Microtuberization in potato (Solanum tuberosum L.). Plant Cell Report 17(10): 794-798. https://doi.org/10.1007/s002990050485
Instituto Nacional de Estadística e Informática (INEI). (2012). IV Censo Nacional Agropecuario (CNA). Available in: http://proyectos.inei.gob.pe/web/DocumentosPublicos/ResultadosFinalesIVCENAGRO.pdf [2017, august 24]
Jiménez, E., Pérez, N., de Feria, M., Barbón, R., Capote, A., Chávez, M., Quiala, E. and Pérez. J.C. (1999). Improved production of potato microtubers using a temporary immersion system. Plant Cell, Tissue and Organ Culture. 59(1): 19-23. https://doi.org/10.1023/A:1006312029055
Kämäräinen-Karppinen, T., Virtanen, E., Rokka, V.M. and Pirttilä A.M. (2010). Novel bioreactor technology for mass propagation of potato microtubers. Plant Cell, Tissue and Organ Culture 101(2:245-249. DOI: 10.1007/s11240-010-9679-7
Murashige, T., and Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiología Plantarum 15(3): 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Radouani, A, and Lauer F.I. (2015). Field Performance of Cultivars Nicola and Russet Burbank Micro and Minitubers. American Journal of Potato Research 92(2):298-302. https://doi.org/10.1007/s12230-014-9421-9
Ranalli, P. (1997). Innovative propagation methods used in seed tuber multiplication systems. Potato Research 40(4):439-453. https://doi.org/10.1007/BF02358004
Ranalli, P., Bassi, F., Ruaro, G., Del Re, P., Di Candilo, M. and Mandolino. G. (1994). Microtuber and minituber production and field performance compared with normal tubers. Potato Research 37(4):383-391. https://doi.org/10.1007/BF02358352
Teisson, C, and Alvard. D. (1999). In vitro production of potato microtubers in liquid medium using temporary immersion. Potato Research 42(3-4):499-504. https://doi.org/10.1007/BF02358166
Venkatasalam E.P., Singh, B.P., Pandey, K.K., Latawa, J., Sharma, S., Sood, R. and Thakur V. (2012). Effect of carbon sources and explants on in vitro multiplication of potato. Potato Journal 39(2): 166-172.
