¿EL IBUPROFENO Y FENOBARBITAL INFLUYEN EN LA TOLERANCIA DEL VECTOR DEL DENGUE AL LARVICIDA TEMEFOS?
DOI:
https://doi.org/10.21704/rea.v21i1.1874Palavras-chave:
detoxificación, Aedes aegypti, esterasas, temefos, tolerancia, insecticida, ibuprofeno, fenobarbitalResumo
La liberación de productos farmacéuticos se ha incrementado en ecosistemas terrestres y acuáticos poniendo en riesgo a la biota, pudiendo generar múltiples impactos en los organismos, desde modificar la expresión enzimática hasta el impacto intergeneracional en los organismos expuestos. Este estudio evaluó la influencia del fenobarbital e ibuprofeno sobre tolerancia al insecticida temefos en dos poblaciones de Aedes aegypti, La Esperanza (LE) y Rockefeller (Rock). Las larvas I fueron expuestas a 17.7 μg/ml de ibuprofeno y 200 μg/ml de fenobarbital hasta alcanzar el estadio III; posteriormente, se determinó la mortalidad larvaria al temefos (0.005, 0.025 y 0.050 μg/ml) y la actividad enzimática de las esterasas. Se encontró que el fenobarbital favorece una mayor tolerancia a 0.025 μg/ml de temefos, a las 24 horas de exposición, en la población LE (44.00 ± 6.93% de mortalidad) a diferencia de la Rock (97.33 ± 2.67% de mortalidad); además, disminuye la actividad enzimática de las alfa esterasas en los especímenes Rock y LE (0.3892 ± 0.0756 y 0.1722 ± 0.0194, densidad óptica, respectivamente). Asimismo, el ibuprofeno reporta una menor DL90 (0.024 μg/ml temefos) que el testigo (DL90 = 0.039 μg/ml de temefos) a las 2 horas de exposición. Se concluye que el fenobarbital aumenta la tolerancia de larvas de Aedes aegypti al temefos, y el ibuprofeno estimula la actividad de las alfa y beta-esterasas.
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Direitos de Autor (c) 2022 Richar J. Morales-Rodríguez, Judith Roldán-Rodríguez , Karina Salvador-Herrera
Este trabalho encontra-se publicado com a Licença Internacional Creative Commons Atribuição-NãoComercial 4.0.
