The Effects of Benzyladenine and Tomato (Solanum Lycopersicum L.) Extract Concentrations on Shoot Induction of Cattleya sp. Orchid Under In Vitro Culture
DOI:
https://doi.org/10.21704/pja.v8i1.2071Keywords:
Cattleya sp., benzyladenin, Solanum lycopercicum, In vitro, budAbstract
The research aimed to investigate the effects of tomato extract and benzyladenine (BA) concentrations on the in vitro shoot growth of Cattleya sp. The study was carried out from March to July 2023 at the Tissue Culture Laboratory of the Hidayatul Islam Foundation, Medan, Indonesia. Four tomato extract concentrations (0 %, 5 %, 10 %, and 15 %) and three BA concentrations (0 ppm, 2 ppm, and 4 ppm) were employed based on the factorial Complete Randomized Design (CRD). Parameters observed include time of shoot emergence, number of shoots, number of leaves, plantlet height, number of roots, and time of root emergence. The Analysis of Variance (ANOVA) followed by the Tukey Multiple Comparison at α = 0.05 was applied for the statistical analysis. The result showed that the fastest shoot emergence was found in B4T0 (BA ppm and 0 % tomato extract) treatment at 2 weeks after planting (WAP). Treatment B2T10 (2 ppm BA and 10 % tomato extract) had the highest number of leaves and shoots, 5 shoots and 17.67 leaves respectively. The highest plantlet was found in the B4T0 treatment (3.30 cm). This treatment also showed the fastest root emergence and the largest number of roots (5 WAP and 7 roots respectively). In conclusion, treatment B4T10 (4 ppm BA and 10 % tomato extract) was the most potential combination for Cattleya sp. in vitro propagation.
Downloads
References
Agriani, S. M., (2010). Effect of Sweet Potato Extract Concentration and Fish Emulsion on the Growth of PLB of Orchid Cross Phalaenopsis pinlong Cinderella x Vanda tricolor on Knudson Medium C. Fakultas Pertanian. Universitas Sebelas Maret, Surakarta.
Akbar, A., Faridah, E., Indrioko, S., & Herawan, T. S., (2017). Induksi tunas, multiplikasi dan perakaran Gyrinops versteegii (Gilg.) Domke secara in vitro. Jurnal Pemuliaan Tanaman Hutan, 11(1), https://doi.org/10.20886/jpth.2017.11.1.1-13
Annas, M. F. (2019). Effect of 6-Benzyladenine on the growth response of black pomegranate (Punica granatum L.) seeds using the cutting method in Vitro [Doctoral dissertation, Universitas Islam Negeri Maulana Malik Ibrahim]. http://etheses.uin-malang.ac.id/17219/
Apriani, R., Mulyaningsih, T., Kurnianingsih, R., & Fitrahtunnisa (2016). Penggunaan BA Pada Mikropropagasi Pisang (Musa paradisiaca L.) Kultivar Kusto. Biologi Tropis 16(1), 25–32. https://doi.org/10.29303/jbt.v16i1.213
Atiek, M. F., Nurcahyani, E., & Irawan, B. (2022). Vegetative Growth of Potato (Solanum tuberosum L.) Atlantic Cultivar Explants on Murashige and Skoog Medium with the Addition of Tomato Extract (Solanum lycopersicum L.) In Vitro. Jurnal Bioterdidik: Wahana Ekspresi Ilmiah, 10(3), 189–195.
Bhojwani dan Razdan, 1983. Plant Tissue Cultur Theori and Practices. Elvisier Science Publishing Company Inc. New Yorkarroroh
Barroroh, U. & Umul, A. (2005). Effect of Tomato Extract Type and Concentration on the Growth of Cattleya Orchid in vitro. Jurnal Planta Tropika, 1(2), 79–83. https://doi.org/10.18196/pt.v1i2.3122
Dewi, L. K., Nurcahyani, E., Zulkifli, Z., & Lande, M. L. (2021). Effects of tomato (Solanum lycopersicum L.) extract on carbohydrate content and growth of Dendrobium striaenopsis orchid planlets. Agritrop: Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science), 19(1), 67–73. https://doi.org/10.32528/agritrop.v19i1.5473
Dwiyani, R., Aziz, P., Ari, I. & Endang, S. (2009). Increased Growth Rate of Orchid Embryos Vanda tricolor Lindl. on Medium Enriched with Tomato Extract. Proceedings of XX National Biology Seminar. UIN-Malang: 590–597.
Harahap, F., 2012, Fisiologi Tumbuhan: Suatu Pengantar. Medan:Unimed Press, hal 77–88.
Harahap, F., Hariyadi, I., Silitonga, M., Suryani, C., Edi, S., & Ningsih, A. P., (2023). In Vitro Growth of Cattleya Sp Orchid From Leaf Explants with Growth Regulators, Nukleus, 9(1), 192–200. https://doi.org/10.36987/jpbn.v9i1.3945
Hartman, H. T., Kester, D. E., Davies, F. T. & Geneve, R. L. (2010). Plan Propagation Principles and Practices. Prentice Hall Inc, New Jersey.
Heriansyah, P., & Indrawanis, E. (2020). Test of contamination level of orchid explants Bromheadia finlysoniana L. miq in in-vitro culture with addition of tomato extract. Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan,18(1), 223–232. https://doi.org/10.32663/ja.v18i2.1502
Karyaningtyas, A. W., Lestari, A. & Sandra, E., (2023). Pengaruh beberapa formulasi sitokinin terhadap penyediaan bibit dan pertumbuhan eksplan tanaman Pule Pandak (Rauvolfia serpentina (L.) Benth. Ex Kurz) secara in vitro. Agroplasma, 10(1), 237–251
Kartiman, R., Sukma, D., Aisyah, S., & Purwito, A. (2018). In Vitro Multiplication of Black Orchid (Coelogyne pandurata L.) on Combination Treatment of NAA and BAP. Jurnal Bioteknologi & Biosains Indonesia, 5(1), 75–87.
Kieber, J. J., & Schaller, G.A., (2018). Cytokinin signaling in plant development. Development, 145(4), 149344. https://doi.org/10.1242/dev.149344
Maharani, A., Suwirmen & Noli, Z. A. (2018). Effect of gibberellin (GA3) concentration on the growth of kailan (Brassica oleracea L. Var alboglabra) on various growing media with hydroponic wick system. Jurnal Biologi Universitas Andalas, 6(2), 2302–2162. https://doi.org/10.25077/jbioua.6.2.63-70.2018
Nazir, U., Gul, Z., Shah, G. M. & Khan, N. I. (2022). Interaction effect of auxin and cytokinin on in vitro shoot regeneration and rooting of endangered medicinal plant Valeriana jatamansi jones through tissue culture. American Journal of Plant Sciences, 13(02), 223–240. https://doi.org/10.4236/ajps.2022.132014
Ningsih, E. P., & Rohmawati, I. (2019). Response of shoot cuttings of Miana (Coleus atropurpureus (L.) Benth) to the application of growth regulators. Jurnal Biologi Tropis, 19(2), 277–281. https://doi.org/10.29303/jbt.v19i2.1246
Novianti, E., Listiawati, A., & Asnawati, A. (2018). Growth Response of Orchid Cattleya sp. After Acclimatization to the Application of Various Concentrations of Liquid Organic Fertilizer. Jurnal Sains Pertanian Equator, 8(1).
Nurilmala, F. (2018). Textbook of tissue culture. Universitas Nusa Bangsa.
Nurkapita, N., Linda, R., & Zakiah, Z. (2021). Multiplication of Black Orchid (Coelogyne pandurata Lindl.) Shoot Explants with the Addition of NAA (Naphthalene Acetic Acid) and Corn Seed Extract (Zea mays) In Vitro. Jurnal Bios Logos, 11(2), 114–121.
Oktaviana, M. A., & Riza Linda, M. (2015). Growth of Pineapple Crown Shoots (Ananas comosus (L.) Merr) In Vitro with the Addition of Tomato Extract (Solanum lycopersicum L.) and Benzyl Amino Purin (BAP). Protobiont, 4(3).
Rugayah, Suherni, D., Cahya Ginting, Y., & Karyanto, A. (2021). Effect of Red Onion and Tomato Extract Concentration on Mangosteen (Garcinia mangostana L.) Seedling Growth. Jurnal Hortikultura Indonesia, 12(1), 42–50. https://doi.org/10.29244/jhi.12.1.42-50
Saepudin, A., Yulianto, Y., & Aeni, R. N. (2020). In vitro explant growth of dendrobium hybrid orchids on several base media and coconut water concentrations. Media Pertanian, 5(2). https://doi.org/10.37058/mp.v5i2.2451
Sari, R., Asri P. P., Ramadanil, P., Swastika, I. N., (2019). Callus Induction of Dombu Potato Plants (Solanum tuberosum L.) In Vitro with the Addition of Tomato Extract and Coconut Water. Natural Science: Journal of Science and Technology, 8(1), 20–27. https://doi.org/10.22487/25411969.2019.v8.i1.12632
Serliana, M., & Linda, R. (2017). Growth of Black Orchid (Coelogyne pandurata Lindl.) in Vitro with the Addition of Tomato Extract (Solanum lycopersicum L.) and Benzyl Amino Purine (BAP). Jurnal Protobiont, 6(3). http://dx.doi.org/10.26418/protobiont.v6i3.22500
Setiawati, T., Zahra, A., Budiono, R., & Nurzaman, M. (2018). In vitro propagation of potato (solanum tuberosum L. Cv. Granola) by addition of meta-topolin on modified ms (murashige & skoog) media. Jurnal Metamorfosa, 5(1), 44–50.
Syamsiah, M., Imansyah, A. A., Suprapti, H. K., & Badriah, D. S. (2020). Multiplication Response of Moon Orchid (Phalaenopsis sp.) to the Addition of Several Concentrations of BAP (Benzyl Amino Purine) in In Vitro Media. Agroscience, 10(2), 148–159. https://doi.org/10.35194/agsci.v10i2.1157
Tobing, O. (2019). Effectiveness of Benzyl Amino Purin (Bap) and Tomato Extract on the Growth of Dendrobium lineale Orchid on Vacin and Went Medium.
Wijaya, N. R., & Sudrajad, H. (2019). Acceleration of Echinacea purpurea L. Moench shoot growth by benzyl adenine and indole butyric acid addition. Planta Tropika, 7(2), 117–124.
Yachya, A., Andriani, V., & Pramana, Y. B. (2022). Micropropagation of Sulawesi Endemic Plant, Deer Horn Alocasia (Alocasia Jacklyn Sp) Through Shoot and Root Induction. Jurnal Perbenihan Tanaman Hutan, 10(1), 37–46.
Yusnita, Y., Riniarti, M., & Hapsoro, D. (2019). Effect of Activated Charcoal Benzyladenine, and Kinetin on the Growth of Solomon’s Teak (Tectona Grandis Linn. F) Shoots in Vitro. Jurnal Biologi Eksperimen dan Keanekaragaman Hayati, 5(2), 21–30.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Fauziyah Harahap, Amna Kamran Br Tarigan, Cicik Suriani, Abdul Hakim Daulae, Nusyirwan, Syahmi Edi, Elimasni, Nurhasanah, Rifa Fadhillah Munifah Hasibuan
This work is licensed under a Creative Commons Attribution 4.0 International License.
