Los efectos de las concentraciones de benciladenina y extracto de tomate (Solanum lycopersicum L.) sobre la inducción de brotes de Cattleya sp. orquídea bajo cultivo in vitro

Autores/as

  • Fauziyah Harahap Universitas Negeri Medan, Mathematics and Natural Sciences Faculty, Department of Biology, Willem Iskandar Street, Medan, North Sumatra 20221, Indonesia. https://orcid.org/0000-0003-0860-5736
  • Amna Kamran Br Tarigan Universitas Negeri Medan, Mathematics and Natural Sciences Faculty, Department of Biology, Willem Iskandar Street, Medan, North Sumatra 20221, Indonesia.
  • Cicik Suriani Universitas Negeri Medan, Mathematics and Natural Sciences Faculty, Department of Biology, Willem Iskandar Street, Medan, North Sumatra 20221, Indonesia.
  • Abdul Hakim Daulae Universitas Negeri Medan, Mathematics and Natural Sciences Faculty, Department of Biology, Willem Iskandar Street, Medan, North Sumatra 20221, Indonesia.
  • Nusyirwan Universitas Negeri Medan, Mathematics and Natural Sciences Faculty, Department of Biology, Willem Iskandar Street, Medan, North Sumatra 20221, Indonesia.
  • Syahmi Edi Universitas Negeri Medan, Mathematics and Natural Sciences Faculty, Department of Biology, Willem Iskandar Street, Medan, North Sumatra 20221, Indonesia.
  • Elimasni Universitas Sumatera Utara, Faculty of Mathematics and Natural Sciences, Department of Biology, Biotechnology Street, Medan, North Sumatra 20155, Indonesia.
  • Nurhasanah Universitas Mulawarman, Faculty of Agriculture, Department of Agroecotechnology, Pasir Balengkong Street, Samarinda, East Kalimantan 75119, Indonesia.
  • Rifa Fadhillah Munifah Hasibuan Tokyo University of Agriculture and Technology, International Environmental Agricultural Science, Tokyo-Fuchu-Harumicho, 3-Chrome 8-1, Tokyo, Japan.

DOI:

https://doi.org/10.21704/pja.v8i1.2071

Palabras clave:

Cattleya sp., benzyladenin, Solanum lycopercicum, In vitro, brotes

Resumen

Este trabajo tuvo como objetivo investigar los efectos del extracto de tomate y las concentraciones de benciladenina (BA) en el crecimiento de brotes in vitro de Cattleya sp. El estudio se llevó a cabo de marzo a julio de 2023 en el Laboratorio de Cultivo de Tejidos de la Fundación Hidayatul Islam, Medan, Indonesia. Se emplearon cuatro concentraciones de extracto de tomate (0 %, 5 %, 10 %, 15 %) y tres concentraciones de BA (0 ppm, 2 ppm, 4 ppm) con base en el diseño factorial completo aleatorizado (CRD). Los parámetros observados incluyen el tiempo de aparición de los brotes, el número de brotes, el número de hojas, la altura de las plántulas, el número de raíces y el momento de la aparición de las raíces. Para el análisis estadístico se aplicó el Análisis de Varianza (ANOVA) seguido de la Comparación Múltiple de Tukey a α = 0.05. El resultado mostró que la emergencia de brotes más rápida se encontró en el tratamiento B4T0 (BA ppm y 0 % extracto de tomate) a las 2 semanas después de la siembra (WAP). El tratamiento B2T10 (2 ppm de BA y 10 % de extracto de tomate) tuvo el mayor número de hojas y brotes, 5 brotes y 17.67 hojas respectivamente. La plántula más alta se encontró en el tratamiento B4T0 (3.30 cm). Este tratamiento también mostró la emergencia de raíces más rápida y el mayor número de raíces (5 WAP y 7 raíces respectivamente). En conclusión, el tratamiento B4T10 (4 ppm de BA y 10 % de extracto de tomate) fue la combinación más potencial para Cattleya sp. propagación in vitro.

Descargas

Los datos de descarga aún no están disponibles.

Referencias

Agriani, S. M., (2010). Effect of Sweet Potato Extract Concentration and Fish Emulsion on the Growth of PLB of Orchid Cross Phalaenopsis pinlong Cinderella x Vanda tricolor on Knudson Medium C. Fakultas Pertanian. Universitas Sebelas Maret, Surakarta.

Akbar, A., Faridah, E., Indrioko, S., & Herawan, T. S., (2017). Induksi tunas, multiplikasi dan perakaran Gyrinops versteegii (Gilg.) Domke secara in vitro. Jurnal Pemuliaan Tanaman Hutan, 11(1), https://doi.org/10.20886/jpth.2017.11.1.1-13

Annas, M. F. (2019). Effect of 6-Benzyladenine on the growth response of black pomegranate (Punica granatum L.) seeds using the cutting method in Vitro [Doctoral dissertation, Universitas Islam Negeri Maulana Malik Ibrahim]. http://etheses.uin-malang.ac.id/17219/

Apriani, R., Mulyaningsih, T., Kurnianingsih, R., & Fitrahtunnisa (2016). Penggunaan BA Pada Mikropropagasi Pisang (Musa paradisiaca L.) Kultivar Kusto. Biologi Tropis 16(1), 25–32. https://doi.org/10.29303/jbt.v16i1.213

Atiek, M. F., Nurcahyani, E., & Irawan, B. (2022). Vegetative Growth of Potato (Solanum tuberosum L.) Atlantic Cultivar Explants on Murashige and Skoog Medium with the Addition of Tomato Extract (Solanum lycopersicum L.) In Vitro. Jurnal Bioterdidik: Wahana Ekspresi Ilmiah, 10(3), 189–195.

Bhojwani dan Razdan, 1983. Plant Tissue Cultur Theori and Practices. Elvisier Science Publishing Company Inc. New Yorkarroroh

Barroroh, U. & Umul, A. (2005). Effect of Tomato Extract Type and Concentration on the Growth of Cattleya Orchid in vitro. Jurnal Planta Tropika, 1(2), 79–83. https://doi.org/10.18196/pt.v1i2.3122

Dewi, L. K., Nurcahyani, E., Zulkifli, Z., & Lande, M. L. (2021). Effects of tomato (Solanum lycopersicum L.) extract on carbohydrate content and growth of Dendrobium striaenopsis orchid planlets. Agritrop: Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science), 19(1), 67–73. https://doi.org/10.32528/agritrop.v19i1.5473

Dwiyani, R., Aziz, P., Ari, I. & Endang, S. (2009). Increased Growth Rate of Orchid Embryos Vanda tricolor Lindl. on Medium Enriched with Tomato Extract. Proceedings of XX National Biology Seminar. UIN-Malang: 590–597.

Harahap, F., 2012, Fisiologi Tumbuhan: Suatu Pengantar. Medan:Unimed Press, hal 77–88.

Harahap, F., Hariyadi, I., Silitonga, M., Suryani, C., Edi, S., & Ningsih, A. P., (2023). In Vitro Growth of Cattleya Sp Orchid From Leaf Explants with Growth Regulators, Nukleus, 9(1), 192–200. https://doi.org/10.36987/jpbn.v9i1.3945

Hartman, H. T., Kester, D. E., Davies, F. T. & Geneve, R. L. (2010). Plan Propagation Principles and Practices. Prentice Hall Inc, New Jersey.

Heriansyah, P., & Indrawanis, E. (2020). Test of contamination level of orchid explants Bromheadia finlysoniana L. miq in in-vitro culture with addition of tomato extract. Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan,18(1), 223–232. https://doi.org/10.32663/ja.v18i2.1502

Karyaningtyas, A. W., Lestari, A. & Sandra, E., (2023). Pengaruh beberapa formulasi sitokinin terhadap penyediaan bibit dan pertumbuhan eksplan tanaman Pule Pandak (Rauvolfia serpentina (L.) Benth. Ex Kurz) secara in vitro. Agroplasma, 10(1), 237–251

Kartiman, R., Sukma, D., Aisyah, S., & Purwito, A. (2018). In Vitro Multiplication of Black Orchid (Coelogyne pandurata L.) on Combination Treatment of NAA and BAP. Jurnal Bioteknologi & Biosains Indonesia, 5(1), 75–87.

Kieber, J. J., & Schaller, G.A., (2018). Cytokinin signaling in plant development. Development, 145(4), 149344. https://doi.org/10.1242/dev.149344

Maharani, A., Suwirmen & Noli, Z. A. (2018). Effect of gibberellin (GA3) concentration on the growth of kailan (Brassica oleracea L. Var alboglabra) on various growing media with hydroponic wick system. Jurnal Biologi Universitas Andalas, 6(2), 2302–2162. https://doi.org/10.25077/jbioua.6.2.63-70.2018

Nazir, U., Gul, Z., Shah, G. M. & Khan, N. I. (2022). Interaction effect of auxin and cytokinin on in vitro shoot regeneration and rooting of endangered medicinal plant Valeriana jatamansi jones through tissue culture. American Journal of Plant Sciences, 13(02), 223–240. https://doi.org/10.4236/ajps.2022.132014

Ningsih, E. P., & Rohmawati, I. (2019). Response of shoot cuttings of Miana (Coleus atropurpureus (L.) Benth) to the application of growth regulators. Jurnal Biologi Tropis, 19(2), 277–281. https://doi.org/10.29303/jbt.v19i2.1246

Novianti, E., Listiawati, A., & Asnawati, A. (2018). Growth Response of Orchid Cattleya sp. After Acclimatization to the Application of Various Concentrations of Liquid Organic Fertilizer. Jurnal Sains Pertanian Equator, 8(1).

Nurilmala, F. (2018). Textbook of tissue culture. Universitas Nusa Bangsa.

Nurkapita, N., Linda, R., & Zakiah, Z. (2021). Multiplication of Black Orchid (Coelogyne pandurata Lindl.) Shoot Explants with the Addition of NAA (Naphthalene Acetic Acid) and Corn Seed Extract (Zea mays) In Vitro. Jurnal Bios Logos, 11(2), 114–121.

Oktaviana, M. A., & Riza Linda, M. (2015). Growth of Pineapple Crown Shoots (Ananas comosus (L.) Merr) In Vitro with the Addition of Tomato Extract (Solanum lycopersicum L.) and Benzyl Amino Purin (BAP). Protobiont, 4(3).

Rugayah, Suherni, D., Cahya Ginting, Y., & Karyanto, A. (2021). Effect of Red Onion and Tomato Extract Concentration on Mangosteen (Garcinia mangostana L.) Seedling Growth. Jurnal Hortikultura Indonesia, 12(1), 42–50. https://doi.org/10.29244/jhi.12.1.42-50

Saepudin, A., Yulianto, Y., & Aeni, R. N. (2020). In vitro explant growth of dendrobium hybrid orchids on several base media and coconut water concentrations. Media Pertanian, 5(2). https://doi.org/10.37058/mp.v5i2.2451

Sari, R., Asri P. P., Ramadanil, P., Swastika, I. N., (2019). Callus Induction of Dombu Potato Plants (Solanum tuberosum L.) In Vitro with the Addition of Tomato Extract and Coconut Water. Natural Science: Journal of Science and Technology, 8(1), 20–27. https://doi.org/10.22487/25411969.2019.v8.i1.12632

Serliana, M., & Linda, R. (2017). Growth of Black Orchid (Coelogyne pandurata Lindl.) in Vitro with the Addition of Tomato Extract (Solanum lycopersicum L.) and Benzyl Amino Purine (BAP). Jurnal Protobiont, 6(3). http://dx.doi.org/10.26418/protobiont.v6i3.22500

Setiawati, T., Zahra, A., Budiono, R., & Nurzaman, M. (2018). In vitro propagation of potato (solanum tuberosum L. Cv. Granola) by addition of meta-topolin on modified ms (murashige & skoog) media. Jurnal Metamorfosa, 5(1), 44–50.

Syamsiah, M., Imansyah, A. A., Suprapti, H. K., & Badriah, D. S. (2020). Multiplication Response of Moon Orchid (Phalaenopsis sp.) to the Addition of Several Concentrations of BAP (Benzyl Amino Purine) in In Vitro Media. Agroscience, 10(2), 148–159. https://doi.org/10.35194/agsci.v10i2.1157

Tobing, O. (2019). Effectiveness of Benzyl Amino Purin (Bap) and Tomato Extract on the Growth of Dendrobium lineale Orchid on Vacin and Went Medium.

Wijaya, N. R., & Sudrajad, H. (2019). Acceleration of Echinacea purpurea L. Moench shoot growth by benzyl adenine and indole butyric acid addition. Planta Tropika, 7(2), 117–124.

Yachya, A., Andriani, V., & Pramana, Y. B. (2022). Micropropagation of Sulawesi Endemic Plant, Deer Horn Alocasia (Alocasia Jacklyn Sp) Through Shoot and Root Induction. Jurnal Perbenihan Tanaman Hutan, 10(1), 37–46.

Yusnita, Y., Riniarti, M., & Hapsoro, D. (2019). Effect of Activated Charcoal Benzyladenine, and Kinetin on the Growth of Solomon’s Teak (Tectona Grandis Linn. F) Shoots in Vitro. Jurnal Biologi Eksperimen dan Keanekaragaman Hayati, 5(2), 21–30.

Descargas

Publicado

2024-04-29

Número

Vol. 8 Núm. 1 (2024): Enero a Aril

Sección

Artículos

Licencia

Derechos de autor 2024 Fauziyah Harahap, Amna Kamran Br Tarigan, Cicik Suriani, Abdul Hakim Daulae, Nusyirwan, Syahmi Edi, Elimasni, Nurhasanah, Rifa Fadhillah Munifah Hasibuan

Creative Commons License

Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.

Cómo citar

Harahap, F., Br Tarigan, A. K. ., Suriani, C., Hakim Daulae, A. ., Nusyirwan, Syahmi , S. ., Elimasni, Nurhasanah, & Munifah Hasibuan, R. F. . (2024). Los efectos de las concentraciones de benciladenina y extracto de tomate (Solanum lycopersicum L.) sobre la inducción de brotes de Cattleya sp. orquídea bajo cultivo in vitro. Peruvian Journal of Agronomy, 8(1), 44-54. https://doi.org/10.21704/pja.v8i1.2071