Tecnología de cultivo de tejidos de bajo coste para la regeneración de orquídeas
DOI:
https://doi.org/10.21704/pja.v6i2.1360Palabras clave:
gente gelificante alternativo, plántulas de Cattleya, micropropagación, subcultivo de orquídeasResumen
Orchids are one of the most important flowers grown in Sri Lanka for exports and local market. One of the majors constrains in expanding the orchid cultivation is a limited supply of quality planting materials. Micropropagation techniques can be successfully applied for the mass propagation of orchids. But micropropagation is expensive, thus increasing the cost of production. Therefore, this research was undertaken to replacement of expensive agar for in vitro subculturing of Cattaleya seedlings. Two-month-old Cattleya seedlings were subcultures on Murashige and Skoog media (MS) and Knudson C media (KNC) as basal media. As gelling agents’ sago, semolina, corn flour, semolina + agar, corn flour + agar were used. Control treatment was carried out with agar. The cultures were maintained inside the culture room. The number of leaves, number of roots, leaf length, leaf width and percentage of contamination were recorded at four-week intervals. Sago and semolina + agar showed higher performance and low contamination percentage as alternative gelling agents in the MS media. Sago, corn flour, sago + agar, semolina + agar and corn flour + agar showed higher performances and low contamination percentages in KNC media. The results of this study propose that sago, semolina, and corn flour can be used as alternative gelling agents in MS and KNC media for subculture practices of orchids.
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Derechos de autor 2021 P.U. Kumara, HMI Herath, DLCK Fonseka , KKIU Aruna Kumara, AGKKMW Atapattu, FMMT Marikar
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
