Control of avocado root rot caused by Phytophthora cinnamomi with different Trichoderma strains at Chavimochic Irrigation Project


  • Paul Gastañadui Universidad Nacional Agraria La Molina. Facultad de Agronomía, La Molina, Lima, Perú
  • Rocío Moreno Arato Peru S.A. Trujillo, Perú, Lima, Perú.
  • Patricia Elena Quiroz-Delgado Universidad Nacional Agraria La Molina. Facultad de Agronomía, La Molina, Lima, Perú
  • Walter Eduardo Apaza-Tapia Universidad Nacional Agraria La Molina. Facultad de Agronomía, La Molina, Lima, Perú



root rot, biological control, Phytophthora cinnamomi, avocado, Trichoderma


Avocado root rot caused by Phytophthora cinnamomi is one of the main problems affecting avocado (Persea americana) cultivation in Peru, especially at the Chavimochic Irrigation Project. The objective of this study was to evaluate the effect of different Trichoderma strains on the control of Phytophthora cinnamomi in Zutano rootstock under greenhouse conditions. Five isolates of Trichoderma were tested: Trichoderma sp. (Chav01); Trichoderma harzianum (Chavo2); Trichoderma harzianum (UNALM01); Trichoderma viride (UNALM02); and a commercial strain of Trichoderma sp. Evaluations were performed at 30, 45, and 60 days. All isolates colonized the rhizosphere of the avocado. No relation was found between the formation of more Trichoderma colonies and Phytophthora improved control. All strains controlled the root rot, but Chav01 and Chav02 showed the greatest diameter of stem, dry matter in the root, and percentage of healthy root in comparison with UNALM01, UNALM02, and the commercial strain. Thus, the native isolates of Trichoderma from the Chavimochic area can be added to the list of potential new Trichoderma species to control Phytophthora cinnamomi.


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Bae, H., Roberts, D. P., Lim, H. S., Strem, M. D., Park, S. C., Ryu, C. M., Melnick, R. L., & Bailey, B. A. (2011). Endophytic Trichoderma isolates from tropical environments delay disease onset and induce resistance against Phytophthora capsici in hot pepper using multiple mechanisms. Molecular Plant–Microbe Interactions, 24(3), 336–351.

Bae, S.-J., Mohanta, T. K., Chung, J. Y., Ryu, M., Park, G., Shim, S., Hong, S.-B., Seo, H., Bae, D.-W., Bae, I., Kim, J.-J., & Bae, H. (2016). Trichoderma metabolites as biological control agents against Phytophthora pathogens. Biological Control, 92, 128–138.

Benítez, T., Rincón, A. M., Limón, M. C., & Codón, A. C. (2004). Biocontrol mechanisms of Trichoderma strains. International Microbiology, 7(4), 249–260.

Casale, W. L. (1990). Analysis of suppressive soils and development of biological control methods for Phytophthora root rot of avocado. California Avocado Society 1990 Yearbook, 74, 53–56.

Coffey, M. D. (1987). Phytophthora root rot of avocado—An integrated approach to control in California. California Avocado Society 1987 Yearbook, 71, 121–137.

Duvenhage, J. A., & Kotzé, J. M. (1993). Biocontrol of root rot of avocado seedlings. South African Avocado Growers’ Association Yearbook 1993, 16, 70–72.

Erwin, D. C., & Ribeiro, O. K. (1996). Phytophthora diseases worldwide. American Phytopathological Society.

Finlay, A. R., & McCracken, A. R. (1991). Microbial suppression of Phytophthora cinnamomi. In J. A. Lucas, R. C. Hattock, D. S. Shaw & L. R. Cooke (Eds.), Phytophthora (pp. 383–398). Cambridge University Press.

Gees, R., & Coffey, M. D. (1989). Evaluation of strain of Myrothecium roridum as a potential biocontrol agent against Phytophthora cinnamomi. Phytopathology, 79(10), 1079–1084.

Hardham, A. R., & Blackman, L. M. (2018). Phytophthora cinnamomi. Molecular Plant Pathology, 19(2), 260–285.

Lamari, L. (2008). ASSESS 2.0 Image analysis software for plant disease quantification. American Phytopathological Society, St. Paul.

Mass, E. M. C., & Kotzé, J. M. (1990). The effect of bacteria on root severity caused by Phytophthora cinnamomi. South African Avocado Growers’ Association Yearbook, 13, 65–66.

Mcleod, A., Labuschagne, N., & Kotzé, J. M. (1995). Evaluation of Trichoderma for biological control of avocado root rot in bark medium artificially infested with Phytophthora cinnamomi. South African Avocado Growers’ Association Yearbook, 18, 32–37.

Ploetz, R. C. (2013). Phytophthora root rot of Avocado. In K. Lamour (Ed.), Phytophthora: A Global Perspective (pp. 197–203). CABI Plant Protection Series. CABI Publishing.

Reeves, R. J. (1975). Behaviour of Phytophthora cinnamomi Rands in different soils and water regimes. Soil Biology and Biochemistry, 7(1), 19–24.

Samuels, G. J. (2006). Trichoderma: Systematics, the sexual state, and ecology. Phytopathology, 96(2), 195–206.

Sid Ahmed, A., Pérez‐Sánchez, C., Egea, C., & Candela, M. E. (1999). Evaluation of Trichoderma harzianum for controlling root rot caused by Phytophthora capsici in pepper plants. Plant Pathology, 48(1), 58–65.

Stirling, A. M., Hayward, A. C., & Pegg, K. G. (1992). Evaluation of the biological control potential of bacteria isolated from soil suppressive to Phytophthora cinnamomi. Australasian Plant Pathology, 21(4), 133–142.

Villavicencio, Y. (2018). Comportamiento de cinco patrones de palto (Persea americana Mill.) a Phytophthora cinnamomi Rands en Chavimochic en invernadero [Thesis, Universidad Nacional Agraria la Molina]. UNALM Repository.

Vinale, F., Sivasithamparam, K., Ghisalberti, E. L., Marra, R., Woo, S. L., & Lorito, M. (2008). Trichoderma–plant–pathogen interactions. Soil Biology and Biochemistry, 40(1), 1–10.

Zemtmeyer, G. A. (1985). Origen and distribution of Phytophthora cinnamomi. California Avocado Society 1985 Yearbook, 69, 89–94.